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KMID : 0364820080440040277
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Korean Journal of Microbiology
2008 Volume.44 No. 4 p.277 ~ p.281
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Human Immunodeficiency Virus-1 Tat Positively Regulates the Human CD99 Gene via DNA Demethylation
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Lee Eu-Gene
Kim Ye-Ri
Lee Mi-Kyung
Lee Im-Soon
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Abstract
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HIV affects many organ systems. Patients with HIV infection have substantially increased risk of developing various cancers, primarily by opportunistic infection with oncogenic viruses due to their immunocompromised status. However, extensive evidence also indicates that the viral protein, Tat itself, may play as a major factor in the development of AIDS-related neoplasms. The molecular mechanism underlying Tat’s oncogenic activity may include deregulation of cellular genes. Therefore, in this study, we examined the effect of HIV-1 Tat on CD99 as one of the target cellular genes, which is a well-known tumor marker in several cancers. By using established HeLa clones that are stably expressing Tat, we found that CD99 is upregulated by endogenous Tat, whereas STAT3 is down regulated. Upon the screening of genes differentially expressed between Tat-stable cells and the control cells by using the gene fishing technique, DEG, we detected 3 genes which expression is affected by the presence of Tat. Furthermore, the methylation specific PCR analysis of the stably Tat expressing cell lines revealed that the CD99 promoter is demethylated in the presence of Tat. Taken together, these results open a potential role of CD99 in AIDS-related oncogenesis via epigenetic regulation by HIV-1 Tat.
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KEYWORD
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CD99 promoter, CpG island, HIV-1-Tat, methylation
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